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Thoas Fioretos

Thoas Fioretos

Research team manager

Thoas Fioretos

Single-cell sequencing in translational cancer research and challenges to meet clinical diagnostic needs

Author

  • Ulrich Pfisterer
  • Julia Bräunig
  • Per Brattås
  • Markus Heidenblad
  • Göran Karlsson
  • Thoas Fioretos

Summary, in English

The ability to capture alterations in the genome or transcriptome by next-generation sequencing has provided critical insight into molecular changes and programs underlying cancer biology. With the rapid technological development in single-cell sequencing, it has become possible to study individual cells at the transcriptional, genetic, epigenetic, and protein level. Using single-cell analysis, an increased resolution of fundamental processes underlying cancer development is obtained, providing comprehensive insights otherwise lost by sequencing of entire (bulk) samples, in which molecular signatures of individual cells are averaged across the entire cell population. Here, we provide a concise overview on the application of single-cell analysis of different modalities within cancer research by highlighting key articles of their respective fields. We furthermore examine the potential of existing technologies to meet clinical diagnostic needs and discuss current challenges associated with this translation.

Department/s

  • Center for Translational Genomics (CTG)
  • LUCC: Lund University Cancer Centre
  • Translational Genomic and Functional Studies of Leukemia
  • Division of Clinical Genetics
  • Stem Cells and Leukemia
  • Division of Molecular Hematology (DMH)
  • StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
  • Department of Laboratory Medicine

Publishing year

2021-07-01

Language

English

Pages

504-524

Publication/Series

Genes, Chromosomes and Cancer

Volume

60

Issue

7

Document type

Journal article review

Publisher

John Wiley & Sons Inc.

Topic

  • Cancer and Oncology

Status

Published

Research group

  • Translational Genomic and Functional Studies of Leukemia
  • Stem Cells and Leukemia

ISBN/ISSN/Other

  • ISSN: 1045-2257