
Peter James
Professor

Protein Expression Changes in Ovarian Cancer during the Transition from Benign to Malignant.
Author
Summary, in English
Epithelial ovarian carcinoma has in general a poor prognosis since the vast majority of tumors are genomically unstable and clinically highly aggressive. This results in rapid progression of malignancy potential while still asymptomatic and thus in late diagnosis. It is therefore of critical importance to develop methods to diagnose epithelial ovarian carcinoma at its earliest developmental stage, that is, to differentiate between benign tissue and its early malignant transformed counterparts. Here we present a shotgun quantitative proteomic screen of benign and malignant epithelial ovarian tumors using iTRAQ technology with LC-MALDI-TOF/TOF and LC-ESI-QTOF MS/MS. Pathway analysis of the shotgun data pointed to the PI3K/Akt signaling pathway as a significant discriminatory pathway. Selected candidate proteins from the shotgun screen were further confirmed in 51 individual tissue samples of normal, benign, borderline or malignant origin using LC-MRM analysis. The MRM profile demonstrated significant differences between the four groups separating the normal tissue samples from all tumor groups as well as perfectly separating the benign and malignant tumors with a ROC-area of 1. This work demonstrates the utility of using a shotgun approach to filter out a signature of a few proteins only that discriminates between the different sample groups.
Department/s
- Department of Immunotechnology
- Computational Biology and Biological Physics - Undergoing reorganization
- Infection Medicine Proteomics
Publishing year
2012
Language
English
Pages
2876-2889
Publication/Series
Journal of Proteome Research
Volume
11
Issue
5
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Biophysics
- Immunology in the medical area
Status
Published
Research group
- Infection Medicine Proteomics
ISBN/ISSN/Other
- ISSN: 1535-3893