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Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore.

  • Hamid Niksirat
  • Liselotte Andersson
  • Peter James
  • Antonín Kouba
  • Pavel Kozák
Publishing year: 2014
Language: English
Pages: 335-344
Publication/Series: Animal Reproduction Science
Volume: 149
Issue: 3-4
Document type: Journal article
Publisher: Elsevier

Abstract english

Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization.


  • Immunology in the medical area


  • ISSN: 1873-2232
Peter James
E-mail: peter [dot] james [at] immun [dot] lth [dot] se


Department of Immunotechnology

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