Håkan Axelson
Research team manager
HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex
Author
Summary, in English
The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems.
Department/s
- Department of Translational Medicine
- Division of Molecular Medicine and Gene Therapy
Publishing year
2000
Language
English
Pages
22-31
Publication/Series
Biochemical and Biophysical Research Communications
Volume
274
Issue
1
Document type
Journal article
Publisher
Elsevier
Topic
- Biological Sciences
Keywords
- SEF2-1
- neuroblastoma
- HASH-1
- sympathetic nervous system
- E2-2
- bHLH
- differentiation
- yeast two-hybrid
Status
Published
ISBN/ISSN/Other
- ISSN: 1090-2104