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Håkan Axelson

Håkan Axelson

Research team manager

Håkan Axelson

HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex

Author

  • Paula Persson
  • Annika Jögi
  • Anna Grynfeld
  • Sven Påhlman
  • Håkan Axelson

Summary, in English

The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems.

Department/s

  • Department of Translational Medicine
  • Division of Molecular Medicine and Gene Therapy

Publishing year

2000

Language

English

Pages

22-31

Publication/Series

Biochemical and Biophysical Research Communications

Volume

274

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • SEF2-1
  • neuroblastoma
  • HASH-1
  • sympathetic nervous system
  • E2-2
  • bHLH
  • differentiation
  • yeast two-hybrid

Status

Published

ISBN/ISSN/Other

  • ISSN: 1090-2104