Håkan Axelson
Research team manager
Size-based isolation and detection of renal carcinoma cells from whole blood
Author
Summary, in English
Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.
Department/s
- Division of Translational Cancer Research
- Department of Laboratory Medicine
- Division of Clinical Genetics
Publishing year
2022-03-21
Language
English
Publication/Series
Molecular and clinical oncology
Volume
16
Issue
5
Document type
Journal article
Publisher
Spandidos Publications
Topic
- Cancer and Oncology
Keywords
- circulating tumour cells
- kidney cancer
- liquid biopsy
- molecular diagnostics
Status
Published
ISBN/ISSN/Other
- ISSN: 2049-9450