LIM-only protein Lmo2 forms a protein complex with erythroid transcription factor GATA-1

Summary, in English

The LIM-only protein Lmo2, originally identified as an oncogenic protein in human T cell leukemia, is essential for erythropoiesis. A possible role for Lmo2 in transcription during erythropoiesis has been investigated. Direct interaction of Lmo2 was observed in vitro and in vivo with the zinc finger transcription factor GATA-1, as well as with the basic helix-loop-helix (bHLH) transcription factor Tall. By using mammalian two-hybrid analysis, E47/Tall/Lmo2/GATA-1 protein complex could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis. This data suggest that variations in amounts of complexes involving Lmo2, Tall, and GATA-1 could be important for erythroid differentiation.

• Author: H Osada; G G Grutz; H Axelson; A Forster; T H Rabbitts
• Department/s: Division of Translational Cancer Research
• Publishing year: 1997-04
• Language: English
• Pages: 12-307
• Publication/Series: Leukemia
• Volume: 11
• Issue: Suppl 3
• Document type: Journal article
• Publisher: Nature Publishing Group
• Topic: Hematology
• Keywords: Adaptor Proteins, Signal Transducing; Animals; Basic Helix-Loop-Helix Transcription Factors; COS Cells; DNA-Binding Proteins; Erythroid-Specific DNA-Binding Factors; Erythropoiesis; GATA1 Transcription Factor; Helix-Loop-Helix Motifs; Humans; LIM Domain Proteins; Leukemia, Erythroblastic, Acute; Leukemia, T-Cell; Metalloproteins; Proto-Oncogene Proteins; Recombinant Proteins; Transcription Factors; Transcription, Genetic; Transfection; Tumor Cells, Cultured; Zinc Fingers
• Status: Published
• ISBN/ISSN/Other: ISSN: 0887-6924