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Selective infection of E. coli as a function of a specific molecular interaction.

Author:
  • Nina Nilsson
  • Fredrik Karlsson
  • Jasna Rakonjac
  • Carl Borrebaeck
Publishing year: 2002
Language: English
Pages: 27-32
Publication/Series: Journal of Molecular Recognition
Volume: 15
Issue: 1
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries.

Keywords

  • Immunology in the medical area
  • Protein Engineering
  • Peptide Library
  • Escherichia coli Infections/*microbiology
  • Bacteriophages/metabolism/*physiology
  • Escherichia coli/metabolism/*physiology
  • Support
  • Non-U.S. Gov't
  • U.S. Gov't
  • Non-P.H.S.

Other

Published
  • ISSN: 1099-1352
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

Professor

Department of Immunotechnology

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Director

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