Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.

Summary, in English

Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy.

• Author: Sissela Broos; Kristina Lundberg; Takami Akagi; Koji Kadowaki; Mitsuru Akashi; Lennart Greiff; Carl Borrebaeck; Malin Lindstedt
• Department/s: Department of Immunotechnology; Otorhinolaryngology (Lund)
• Publishing year: 2010
• Language: English
• Pages: 5075-5085
• Publication/Series: Vaccine
• Volume: 28
• Links: http://dx.doi.org/10.1016/j.vaccine.2010.05.004
• Document type: Journal article
• Publisher: Elsevier
• Topic: Otorhinolaryngology
• Status: Published
• ISBN/ISSN/Other: ISSN: 1873-2518