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Åke Borg

Åke Borg

Principal investigator

Åke Borg

High-definition spatial transcriptomics for in situ tissue profiling

Author

  • Sanja Vickovic
  • Gökcen Eraslan
  • Fredrik Salmén
  • Johanna Klughammer
  • Linnea Stenbeck
  • Denis Schapiro
  • Tarmo Äijö
  • Richard Bonneau
  • Ludvig Bergenstråhle
  • José Fernandéz Navarro
  • Joshua Gould
  • Gabriel K. Griffin
  • Åke Borg
  • Mostafa Ronaghi
  • Jonas Frisén
  • Joakim Lundeberg
  • Aviv Regev
  • Patrik L. Ståhl
Show all

Summary, in English

Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.

Department/s

  • Familial Breast Cancer
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation

Publishing year

2019

Language

English

Pages

987-990

Publication/Series

Nature Methods

Volume

16

Issue

10

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Medical Laboratory and Measurements Technologies

Status

Published

Research group

  • Familial Breast Cancer

ISBN/ISSN/Other

  • ISSN: 1548-7105