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Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells

Author:
  • H Söderholm
  • Eva Örtoft
  • Irja Johansson
  • June Ljungberg
  • Christer Larsson
  • Håkan Axelson
  • Sven Påhlman
Publishing year: 1999
Language: English
Pages: 557-563
Publication/Series: Biochemical and Biophysical Research Communications
Volume: 256
Issue: 3
Document type: Journal article
Publisher: Elsevier

Abstract english

The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate.

Keywords

  • Biological Sciences

Other

Published
  • Diabetes and Endocrinology
  • ISSN: 1090-2104
Sven Påhlman
E-mail: sven [dot] pahlman [at] med [dot] lu [dot] se

Professor

Division of Translational Cancer Research

+46 46 222 64 21

MV406 312K1

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