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Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Author:
  • Q Wanying
  • T Brodin
  • Crister Ceberg
  • Christian Ingvar
  • Kristina Norrgren
  • H O Sjogren
  • Sven-Erik Strand
Publishing year: 1991
Language: English
Pages: 379-383
Publication/Series: Acta Oncologica
Volume: 30
Issue: 3
Document type: Journal article
Publisher: Taylor & Francis

Abstract english

To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.

Keywords

  • Cancer and Oncology
  • Monoclonal antibodies
  • colon carcinoma
  • rat

Other

Published
  • Medical Radiation Physics, Malmö
  • ISSN: 1651-226X
Sven-Erik Strand
E-mail: sven-erik [dot] strand [at] med [dot] lu [dot] se

Project manager

Systemic Radiation Therapy Group

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Professor emeritus

Medical Radiation Physics, Lund

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