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Peter James

Peter James

Professor

Peter James

Occupational second-hand smoke exposure : A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers

Author

  • Sofia Neves
  • Solange Pacheco
  • Fátima Vaz
  • Peter James
  • Tânia Simões
  • Deborah Penque

Summary, in English

Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.

Department/s

  • Department of Immunotechnology

Publishing year

2024-06

Language

English

Publication/Series

Environmental Toxicology and Pharmacology

Volume

108

Document type

Journal article

Publisher

Elsevier

Topic

  • Cancer and Oncology

Keywords

  • Cigarette smoke
  • Mass Spectrometry
  • Nasal epithelium
  • Protein network
  • Proteomics
  • Second-Hand Smoke

Status

Published

ISBN/ISSN/Other

  • ISSN: 1382-6689