Peter James
Professor
Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus
Author
Summary, in English
Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p < 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232.
Department/s
- Department of Immunotechnology
Publishing year
2017-08
Language
English
Pages
189-193
Publication/Series
Biochemical and Biophysical Research Communications
Volume
490
Issue
2
Document type
Journal article
Publisher
Elsevier
Topic
- Fish and Wildlife Management
- Biochemistry and Molecular Biology
Keywords
- Egg
- Fertilization
- Label-free
- Protein
- Quantitative
Status
Published
ISBN/ISSN/Other
- ISSN: 0006-291X