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Peter James

Peter James

Professor

Peter James

Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus

Author

  • Hamid Niksirat
  • Liselotte Andersson
  • Amin Golpour
  • Latifeh Chupani
  • Peter James

Summary, in English

Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p < 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232.

Department/s

  • Department of Immunotechnology

Publishing year

2017-08

Language

English

Pages

189-193

Publication/Series

Biochemical and Biophysical Research Communications

Volume

490

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Fish and Wildlife Management
  • Biochemistry and Molecular Biology

Keywords

  • Egg
  • Fertilization
  • Label-free
  • Protein
  • Quantitative

Status

Published

ISBN/ISSN/Other

  • ISSN: 0006-291X