Peter James
Professor
Proteomic analysis and discovery using affinity proteomics and mass spectrometry.
Author
Summary, in English
Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept.
Department/s
- Department of Immunotechnology
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2011
Language
English
Publication/Series
Molecular & Cellular Proteomics
Volume
10
Document type
Journal article
Publisher
American Society for Biochemistry and Molecular Biology
Topic
- Immunology in the medical area
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-9484