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Peter James

Peter James

Professor

Peter James

Automated reporting from gel-based proteomics experiments using the open source Proteios database application

Author

  • Fredrik Levander
  • Morten Krogh
  • Kristofer Wårell
  • Per Gärdén
  • Peter James
  • Jari Häkkinen

Summary, in English

The assembly of data from different parts of proteomics workflow is often a major bottleneck in proteomics. Furthermore, there is an increasing demand for the publication of details about protein identifications due to the problems with false-positive and false-negative identifications. In this report, we describe how the open-source Proteios software has been expanded to automate the assembly of the different parts of a gel-based proteomics workflow. In Proteios it is possible to generate protein identification reports that contain all the information currently required by proteomics journals. It is also possible for the user to specify maximum allowed false positive ratios, and reports are automatically generated with the corresponding score cut-offs calculated. When protein identification is conducted using multiple search engines, the score thresholds that correlate to the predetermined error rate are also explicitly calculated for proteins that appear on the result lists of more than one search engine.

Department/s

  • Department of Immunotechnology
  • Computational Biology and Biological Physics - Undergoing reorganization

Publishing year

2007

Language

English

Pages

668-674

Publication/Series

Proteomics

Volume

7

Issue

5

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Immunology in the medical area
  • Biophysics

Keywords

  • 2-D PAGE
  • protein identification
  • reporting

Status

Published

ISBN/ISSN/Other

  • ISSN: 1615-9861