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Automated reporting from gel-based proteomics experiments using the open source Proteios database application

Author:
  • Fredrik Levander
  • Morten Krogh
  • Kristofer Wårell
  • Per Gärdén
  • Peter James
  • Jari Häkkinen
Publishing year: 2007
Language: English
Pages: 668-674
Publication/Series: Proteomics
Volume: 7
Issue: 5
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

The assembly of data from different parts of proteomics workflow is often a major bottleneck in proteomics. Furthermore, there is an increasing demand for the publication of details about protein identifications due to the problems with false-positive and false-negative identifications. In this report, we describe how the open-source Proteios software has been expanded to automate the assembly of the different parts of a gel-based proteomics workflow. In Proteios it is possible to generate protein identification reports that contain all the information currently required by proteomics journals. It is also possible for the user to specify maximum allowed false positive ratios, and reports are automatically generated with the corresponding score cut-offs calculated. When protein identification is conducted using multiple search engines, the score thresholds that correlate to the predetermined error rate are also explicitly calculated for proteins that appear on the result lists of more than one search engine.

Keywords

  • Immunology in the medical area
  • Biophysics
  • 2-D PAGE
  • protein identification
  • reporting

Other

Published
  • ISSN: 1615-9861
Peter James
E-mail: peter [dot] james [at] immun [dot] lth [dot] se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

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