The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Peter James

Peter James

Professor

Peter James

140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry.

Author

  • Tord Berggård
  • Giorgio Arrigoni
  • Olof Olsson
  • Malin Fex
  • Sara Linse
  • Peter James

Summary, in English

Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. After comparing the results from the different experiments, we were able to exclude a significant number of proteins suspected to bind in a nonspecific manner. A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. This study provides a functional framework for studies on these previously uncharacterized proteins.

Department/s

  • Biophysical Chemistry
  • Department of Immunotechnology
  • Pure and Applied Biochemistry
  • Celiac Disease and Diabetes Unit
  • Biochemistry and Structural Biology

Publishing year

2006

Language

English

Pages

669-687

Publication/Series

Journal of Proteome Research

Volume

5

Issue

3

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Basic Medicine

Keywords

  • calcium
  • protein-protein interactions
  • calmodulin
  • proteomics
  • brain

Status

Published

Research group

  • Celiac Disease and Diabetes Unit

ISBN/ISSN/Other

  • ISSN: 1535-3893