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Peter James

Peter James

Professor

Peter James

Protein Expression Changes in Skeletal Muscle in Response to Growth Promoter Abuse in Beef Cattle

Author

  • Roberto Stella
  • Giancarlo Biancotto
  • Morten Krogh
  • Roberto Angeletti
  • Giandomenico Pozza
  • Maria Catia Sorgato
  • Peter James
  • Igino Andrighetto

Summary, in English

The fraudulent treatment of cattle with growth promoting agents (GPAs) is a matter of great concern for the European Union (EU) authorities and consumers. It has been estimated that 10% of animals are being illegally treated in the EU. In contrast, only a much lower percentage of animals (< 0.5%) are actually found as being noncompliant by conventional analytical methods. Thus, it has been proposed that methods should be developed that can detect the use of the substances via the biological effects of these substances on target organs, such as the alteration of protein expression profiles. Here we present a study aimed at evaluating if a correlation exists between the treatment with GPAs and alterations in the two-dimensional electrophoresis (2DE) protein pattern obtained from the biceps brachii skeletal muscle from mixed-bred cattle. After image analysis and statistical evaluation, protein spots that differentiate between treated and control groups were selected for analysis by mass spectrometry. A set of proteins could be defined that accurately detect the use of glucocorticoids and beta(2)-agonists as growth promoters through the changes caused in muscle differentiation. As a further validation, we repeated the analysis using an independent set of samples from a strain of pure-bred cattle and verified these proteins by Western blot analysis.

Department/s

  • Computational Biology and Biological Physics - Has been reorganised
  • Department of Immunotechnology

Publishing year

2011

Language

English

Pages

2744-2757

Publication/Series

Journal of Proteome Research

Volume

10

Issue

6

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Immunology in the medical area

Keywords

  • dexamethasone
  • clenbuterol
  • growth enhancement
  • cattle
  • 2D-PAGE
  • biomarkers

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3893