Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis

Summary, in English

Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.

• Author: Paolo Cifani; Maria Bendz; Kristofer Wårell; Karin M Hansson; Fredrik Levander; Marianne Sandin; Morten Krogh; Marie Ovenberger; Erik Fredlund; Marica Vaapil; Alexander Pietras; Sven Påhlman; Peter James
• Department/s: Department of Immunotechnology; Computational Biology and Biological Physics - Undergoing reorganization; Department of Translational Medicine; Division of Translational Cancer Research; BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
• Publishing year: 2011
• Language: English
• Pages: 1645-1656
• Publication/Series: Journal of Proteome Research
• Volume: 10
• Issue: 4
• Links: http://dx.doi.org/10.1021/pr100982j
• Document type: Journal article
• Publisher: The American Chemical Society (ACS)
• Topic: Biophysics; Immunology in the medical area
• Keywords: isotopic labeling; HPLC-MS; 2D-PAGE; plasma membrane; protein; expression; mRNA expression; correlation; hypoxia; normoxia; neuroblastoma
• Status: Published
• ISBN/ISSN/Other: ISSN: 1535-3893