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Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent)

  • Pedro Aqueveque
  • Carlos L. Céspedes
  • José Becerra
  • Mario Aranda
  • Olov Sterner
Publishing year: 2017-05-18
Language: English
Publication/Series: Food and Chemical Toxicology
Document type: Journal article
Publisher: Elsevier

Abstract english

Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000-2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL.Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides.


  • Analytical Chemistry
  • Pharmacology and Toxicology
  • Antifungal
  • Botrytis
  • Chilean fungi
  • Sterenin D
  • Stereum
  • Submerged cultures


  • ISSN: 0278-6915
Olov Sterner
E-mail: olov [dot] sterner [at] science [dot] lu [dot] se


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