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Håkan Axelson

Håkan Axelson

Research team manager

Håkan Axelson

HIF-1alpha induces MXI1 by alternate promoter usage in human neuroblastoma cells.

Author

  • Tobias Löfstedt
  • Erik Fredlund
  • Rosa Noguera
  • Samuel Navarro
  • Linda Holmquist Mengelbier
  • Siv Beckman
  • Sven Påhlman
  • Håkan Axelson

Summary, in English

Adaptation to low oxygen conditions is essential for maintaining homeostasis and viability in oxygen-consuming multi-cellular tissues, including solid tumors. Central in these processes are the hypoxia-inducible transcription factors, HIF-1 and HIF-2, controlling genes involved in e.g. glucose metabolism and neovascularization. Tumor hypoxia and HIF expression have also been associated with a dedifferentiated phenotype and increased aggressiveness. In this report we show that the MAX interactor-1 (MXI1) gene is directly regulated by HIF proteins in neuroblastoma and breast cancer cells. HIF-binding and transactivation were detected within MXI1 gene regulatory sequences in the vicinity of the MXI1-0 promoter, leading to rapid induction of the alternate MXI1-0 isoform followed by a long-term induction of both the MXI1-0 and MXI1 isoforms. Importantly, knock-down of MXI1 had limited effect on MYC/MYCN activity under hypoxia, an observation that might be related to the different functional attributes of the two MXI1 isoforms.

Department/s

  • Department of Translational Medicine

Publishing year

2009

Language

English

Pages

1924-1936

Publication/Series

Experimental Cell Research

Volume

315

Issue

11

Document type

Journal article

Publisher

Academic Press

Topic

  • Cancer and Oncology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1090-2422