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6;7 chromosomal translocation in spontaneously arising rat immunocytomas : evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target

Author:
  • W S Pear
  • G Wahlström
  • S F Nelson
  • H Axelson
  • A Szeles
  • F Wiener
  • H Bazin
  • G Klein
  • J Sümegi
Publishing year: 1988-01
Language: English
Pages: 51-441
Publication/Series: Molecular and Cellular Biology
Volume: 8
Issue: 1
Document type: Journal article
Publisher: American Society for Microbiology

Abstract english

Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.

Keywords

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • DNA, Neoplasm
  • Gene Expression Regulation
  • Genes, Immunoglobulin
  • Genes, Switch
  • Immunoglobulin Constant Regions
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Isotypes
  • Karyotyping
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Plasmacytoma
  • Proto-Oncogene Proteins
  • Rats
  • Translocation, Genetic

Other

Published
  • ISSN: 0270-7306
Håkan Axelson
E-mail: hakan [dot] axelson [at] med [dot] lu [dot] se

Professor

Division of Translational Cancer Research

+46 46 222 64 34

MV 404 A31A2

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