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Serum proteome profiling of pancreatitis using recombinant antibody microarrays reveals disease-associated biomarker signatures

Author:
  • Anna Sandström Gerdtsson
  • Roland Andersson
  • R. Segersvard
  • M. Lohr
  • Carl Borrebaeck
  • Christer Wingren
Publishing year: 2012
Language: English
Pages: 96-486
Publication/Series: Proteomics Clinical Applications
Volume: 6
Issue: 9-10
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

PURPOSE: Pancreatitis is an inflammatory state of the pancreas, for which high-performing serological biomarkers are lacking. The aim of the present study was to evaluate the use of affinity proteomics for identifying potential markers of disease and stratifying pancreatitis subtypes. EXPERIMENTAL DESIGN: High-content, recombinant antibody microarrays were applied for serum protein expression profiling of 113 serum samples from patients with chronic, acute, and autoimmune pancreatitis, as well as healthy controls. The sample groups were compared using supervised classification based on support vector machine analysis. RESULTS: This discovery study showed that pancreatitis subtypes could be discriminated with high accuracy. Using unfiltered data, the individual subtypes, as well as the combined pancreatitis cohort, were distinguished from healthy controls with high AUC values (0.96-1.00). Moreover, characteristic protein patterns and AUC values in the range of 0.69-0.95 were observed for the individual pancreatitis entities when compared to each other, and to all other samples combined. CONCLUSIONS AND CLINICAL RELEVANCE: This study demonstrated the potential of the antibody microarray approach for stratification of pancreatitis. Distinct candidate multiplex serum biomarker signatures for chronic, acute, and autoimmune pancreatitis were defined, which could enhance our fundamental knowledge of the underlying molecular mechanisms, and potentially lead to improved diagnosis.

Keywords

  • Surgery
  • Retrospective Studies
  • Proteomics
  • Proteome/*analysis
  • *Protein Array Analysis
  • Humans
  • Female
  • Gene Expression Profiling
  • 80 and over
  • Area Under Curve
  • Recombinant Proteins/genetics/immunology/metabolism
  • Biological Markers/blood
  • Adult
  • Aged
  • Adolescent
  • Male
  • Pancreatitis/*blood/pathology
  • Middle Aged
  • Single-Chain Antibodies/genetics/*immunology/metabolism
  • Young Adult

Other

Published
  • CREATE Health
  • ISSN: 1862-8354
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

Professor

Department of Immunotechnology

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Director

Create Health

+46 46 222 96 13

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