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Carl Borrebaeck

Carl Borrebaeck

Professor

Carl Borrebaeck

Human MoAbs produced from normal, HIV-1-negative donors and specific for glycoprotein gp120 of the HIV-1 envelope

Author

  • M. Ohlin
  • J. Hinkula
  • P. A. Broliden
  • R. Grunow
  • C. A K Borrebaeck
  • B. Wahren

Summary, in English

Human MoAbs ofIgM class were developed against three regions of the HIV-1 envelope. Uninfected donor lymphocytes were immunized in vitro with recombinant protein pB1. Four out of five antibodies were directed to different parts of the V3 region, which contains a major neutralizing site. Two out of these antibodies were directed to more than one amino acid sequence, indicating reactivity to discontinuous sites. Two of the human MoAbs inhibited viral spread between cells in tissue culture, interpreted as reactivities to conserved amino acid sequences exposed during viral maturation. No MoAb neutralized virus, which may be explained by the relatively low avidity of the antibodies. One MoAb was directed to a region containing amino acids participating in CD4 binding. This technique appears to allow formation of antibodies with fine specificities other than those obtained in infected hosts.

Department/s

  • Department of Immunotechnology
  • Create Health

Publishing year

1992

Language

English

Pages

290-295

Publication/Series

Clinical and Experimental Immunology

Volume

89

Issue

2

Document type

Journal article

Publisher

British Society for Immunology

Topic

  • Immunology in the medical area

Keywords

  • gp120
  • HIV-1
  • human MoAbs
  • in vitro immunization
  • V3 region

Status

Published

ISBN/ISSN/Other

  • ISSN: 0009-9104