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Identification of a strongly activating human anti-CD40 antibody that suppresses HIV-1 infection

Author:
  • Peter Ellmark
  • Henrik Andersson
  • Sisay Abayneh
  • Eva Maria Fenyö
  • Carl Borrebaeck
Publishing year: 2008
Language: English
Pages: 367-373
Publication/Series: AIDS Research and Human Retroviruses
Volume: 24
Issue: 3
Document type: Journal article
Publisher: Mary Ann Liebert, Inc.

Abstract english

We characterized the functional properties of a novel set of human anti-CD40 monoclonal antibodies originating from a human phage display library and identified an antibody that strongly activates cells via the CD40 receptor for potential use in HIV therapy. The anti-CD40 antibodies were converted from a single chain antibody fragment format (scFv) to an IgG format and produced in HEK293 cells, and the binding characteristics were evaluated. Next, their ability to (1) rescue a human B cell line from induced apoptosis, (2) stimulate B cell proliferation, and (3) block the CD40-CD40L interaction was determined. Finally, the most activating anti-CD40 antibody was tested for its ability to block HIV-1 infection in a monocyte-derived cell line.The different anti-CD40 antibodies, A24, B44, E30, F33, and A2-54, displayed a wide variety of binding and functional properties. In particular, B44 showed a very strong ability to activate normal human B cells and, in addition, did not block the CD40-CD40L interaction. This antibody was able to suppress HIV-1 infection in a human cell line (MonoMac 1) and may be a potential therapeutic candidate in HIV infection.

Keywords

  • Microbiology in the medical area

Other

Published
  • ISSN: 1931-8405
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

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Department of Immunotechnology

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