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Carl Borrebaeck

Carl Borrebaeck

Professor

Carl Borrebaeck

The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

Author

  • Eskil Söderlind
  • Roland Carlsson
  • Carl Borrebaeck
  • Mats Ohlin

Summary, in English

Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.

Department/s

  • Department of Immunotechnology

Publishing year

2001

Language

English

Pages

409-416

Publication/Series

Combinatorial Chemistry & High Throughput Screening

Volume

4

Issue

5

Document type

Journal article

Publisher

Bentham Science Publishers

Topic

  • Immunology in the medical area

Keywords

  • COMPLEMENTARITY-DETERMINING REGIONS
  • PHAGE DISPLAY LIBRARY
  • SINGLE-CHAIN
  • FV
  • AFFINITY HUMAN-ANTIBODIES
  • BY-PASSING IMMUNIZATION
  • RANDOM
  • MUTAGENESIS
  • BINDING-SITE
  • V-H
  • SYNTHETIC REPERTOIRES
  • SOMATIC
  • HYPERMUTATION

Status

Published

ISBN/ISSN/Other

  • ISSN: 1386-2073