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Carl Borrebaeck

Carl Borrebaeck

Professor

Carl Borrebaeck

Affinity and Epitope Profiling of Mouse Anti-CD40 Monoclonal Antibodies

Author

  • Ann-Christin Malmborg Hager
  • Peter Ellmark
  • Carl Borrebaeck
  • Christina Furebring

Summary, in English

The CD40-CD40L interaction plays a critical role in both humoral and cellular immune responses and interfering antibodies have been suggested as an effective approach for the treatment of lymphomas and autoimmune diseases. In this study we have profiled a panel of mouse antihuman CD40 monoclonal antibodies (MoAbs), regarding their CD40 binding affinity and epitope-specificity relative to the CD40L binding in relation to their cellular activating potential. Despite a rather similar domain-recognition profile, the MoAbs blocked the CD40L binding to a varying degree, with MoAb 5C3 being the poorest inhibitor. There was no correlation between affinity and cellular activation potential. In contrast, a correlation between the ability to block CD40L-binding and activation potential could be seen. We believe that this analysis of several mouse anti-CD40 antibodies can be used to develop strategies for producing new human anti-CD40 antibodies that can more effectively induce or block B-cell proliferation.

Department/s

  • Department of Immunotechnology

Publishing year

2003

Language

English

Pages

517-524

Publication/Series

Scandinavian Journal of Immunology

Volume

57

Issue

6

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1365-3083