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Carl Borrebaeck

Carl Borrebaeck

Professor

Carl Borrebaeck

Design of recombinant antibody microarrays for cell surface membrane proteomics

Author

  • Linda Dexlin Mellby
  • Johan Ingvarsson
  • Björn Frendeus
  • Carl Borrebaeck
  • Christer Wingren

Summary, in English

Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

Department/s

  • Department of Immunotechnology

Publishing year

2008

Language

English

Pages

319-327

Publication/Series

Journal of Proteome Research

Volume

7

Issue

1

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3893