Carl Borrebaeck
Professor
Design of recombinant antibody microarrays for cell surface membrane proteomics
Author
Summary, in English
Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.
Department/s
- Department of Immunotechnology
Publishing year
2008
Language
English
Pages
319-327
Publication/Series
Journal of Proteome Research
Volume
7
Issue
1
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Immunology in the medical area
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893