Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Antibody-mediated neutralization of cytomegalovirus: modulation of efficacy induced through the IgG constant region.

Author:
  • Christina Furebring
  • Andrea Speckner
  • Michael Mach
  • Inger Sandlie
  • Lars Norderhaug
  • Carl Borrebaeck
  • Helene Turesson
  • Mats Ohlin
Publishing year: 2002
Language: English
Pages: 833-840
Publication/Series: Molecular Immunology
Volume: 38
Issue: 11
Document type: Journal article
Publisher: Pergamon

Abstract english

Antibodies can neutralize the infectious properties of human cytomegalovirus (CMV). In vivo, the major neutralization determinants are located on glycoprotein B (gB). Recombinant human antibodies, that carry different constant regions (IgG1, IgG3 and the synthetic variant IgG3mA) against two of these epitopes were investigated for their ability to recruit the complement cascade for destruction of the virus. It was shown that all variants of an antibody against the antigenic domain (AD)-2 epitope displayed a similar neutralization activity despite the fact that improved C1q binding was observed for IgG3 and IgG3mA over the IgG1 variant. In contrast, an antibody against the AD-1 epitope carrying the normal IgG3 constant region, was less efficient than its IgG1 counterpart in neutralizing the virus in the absence of complement. However, it restored its activity in the presence of complement to the level of the naturally occurring IgG1 version. The same antibody was substantially more potent in neutralizing the virus in the presence of complement if it carried the IgG3mA constant region. This demonstrates the importance of the constant domain for the biological activity of AD-1 specific antibodies, a factor that should be taken into account when using antibody-based therapeutics or when inducing antibodies by vaccination.

Keywords

  • Immunology in the medical area
  • Neutralization Tests
  • Immunoglobulin G : immunology
  • Molecular Sequence Data
  • Immunoglobulin Constant Region : immunology
  • Human
  • Cytomegalovirus : immunology
  • Complement : physiology
  • Base Sequence
  • Viral : immunology
  • Antibodies
  • Recombinant Proteins : immunology
  • Support
  • Non-U.S. Gov't

Other

Published
  • ISSN: 1872-9142
Carl B
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

Professor

Department of Immunotechnology

313k1

90

Director

Create Health

+46 46 222 96 13

313k1

90