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Carl B

Carl Borrebaeck

Professor

Carl B

Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates

Author

  • Cornelia Steinhauer
  • Anton Ressine
  • György Marko-Varga
  • Thomas Laurell
  • Carl Borrebaeck
  • Christer Wingren

Summary, in English

Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays. (c) 2004 Elsevier Inc. All rights reserved.

Department/s

  • Department of Immunotechnology
  • Department of Biomedical Engineering
  • Centre for Analysis and Synthesis

Publishing year

2005

Language

English

Pages

204-213

Publication/Series

Analytical Biochemistry

Volume

341

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering
  • Analytical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1096-0309