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Carl B

Carl Borrebaeck

Professor

Carl B

From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

Author

  • Sara Ek
  • Ulrika Andreasson
  • S Hober
  • C Kampf
  • F Ponten
  • M Uhlen
  • H Merz
  • Carl Borrebaeck

Summary, in English

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.

Department/s

  • Department of Immunotechnology

Publishing year

2006

Language

English

Pages

1072-1081

Publication/Series

Molecular & Cellular Proteomics

Volume

5

Issue

6

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-9484