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The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

Author:
  • Eskil Söderlind
  • Roland Carlsson
  • Carl Borrebaeck
  • Mats Ohlin
Publishing year: 2001
Language: English
Pages: 409-416
Publication/Series: Combinatorial Chemistry & High Throughput Screening
Volume: 4
Issue: 5
Document type: Journal article
Publisher: Bentham Science Publishers

Abstract english

Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.

Keywords

  • Immunology in the medical area
  • COMPLEMENTARITY-DETERMINING REGIONS
  • PHAGE DISPLAY LIBRARY
  • SINGLE-CHAIN
  • FV
  • AFFINITY HUMAN-ANTIBODIES
  • BY-PASSING IMMUNIZATION
  • RANDOM
  • MUTAGENESIS
  • BINDING-SITE
  • V-H
  • SYNTHETIC REPERTOIRES
  • SOMATIC
  • HYPERMUTATION

Other

Published
  • ISSN: 1386-2073
Carl B
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

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Department of Immunotechnology

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