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Carl B

Carl Borrebaeck

Professor

Carl B

Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics

Author

  • Johan Fransson
  • Sara Ek
  • Peter Ellmark
  • Eskil Söderlind
  • Carl Borrebaeck
  • Christina Furebring

Summary, in English

Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved.

Department/s

  • Department of Immunotechnology

Publishing year

2004

Language

English

Pages

235-242

Publication/Series

Cancer Letters

Volume

208

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Cancer and Oncology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-7980