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Carl B

Carl Borrebaeck

Professor

Carl B

antibody microarray analysis of directly labelled proteomes

Author

  • Christer Wingren
  • Carl Borrebaeck

Summary, in English

In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented.

Department/s

  • Department of Immunotechnology

Publishing year

2008

Language

English

Pages

55-61

Publication/Series

Current Opinion in Biotechnology

Volume

19

Issue

1

Document type

Journal article review

Publisher

Elsevier

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 0958-1669