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Design of recombinant antibody microarrays for cell surface membrane proteomics

Author:
  • Linda Dexlin Mellby
  • Johan Ingvarsson
  • Björn Frendeus
  • Carl Borrebaeck
  • Christer Wingren
Publishing year: 2008
Language: English
Pages: 319-327
Publication/Series: Journal of Proteome Research
Volume: 7
Issue: 1
Document type: Journal article
Publisher: The American Chemical Society

Abstract english

Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

Keywords

  • Immunology in the medical area

Other

Published
  • CREATE Health
  • ISSN: 1535-3893
Carl B
Carl Borrebaeck
E-mail: carl [dot] borrebaeck [at] immun [dot] lth [dot] se

Professor

Department of Immunotechnology

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Director

Create Health

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