
Carl Borrebaeck
Professor

Human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of Neisseria meningitidis
Author
Summary, in English
Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from A. meningitidis B:4:P1,15. The immunized human B cells were Epstein-Barr virus transformed and fused to a heteromyeloma. Several stable human hybridoma cell lines were established and two, secreting antibodies against the 31-kDa class 5c outer membrane protein, were characterized further. The human antibodies were of IgG1 and IgG3 isotypes, with κ light chains. The recognized epitope was commonly found among pathogenic strains of N. meningitidis; thus, these human monoclonal antibodies may be important in the evaluation of N. meningitidis infections.
Department/s
- Department of Immunotechnology
- Division of Microbiology, Immunology and Glycobiology - MIG
Publishing year
1992
Language
English
Pages
1322-1328
Publication/Series
Journal of Infectious Diseases
Volume
166
Issue
6
Document type
Journal article
Publisher
Oxford University Press
Topic
- Infectious Medicine
Status
Published
ISBN/ISSN/Other
- ISSN: 0022-1899