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A glioma classification scheme based on coexpression modules of EGFR and PDGFRA

Author:
  • Yingyu Sun
  • Wei Zhang
  • Dongfeng Chen
  • Yuhong Lv
  • Junxiong Zheng
  • Henrik Lilljebjörn
  • Liang Ran
  • Zhaoshi Bao
  • Charlotte Soneson
  • Hans Olov Sjögren
  • Leif G. Salford
  • Jianguang Ji
  • Pim J. Frenc
  • Thoas Fioretos
  • Tao Jiang
  • Xiaolong Fan
Publishing year: 2014-03-04
Language: English
Pages: 3538-3543
Publication/Series: Proceedings of the National Academy of Sciences of the United States of America
Volume: 111
Issue: 9
Document type: Journal article
Publisher: National Acad Sciences

Abstract english

We hypothesized that key signaling pathways of glioma genesis might enable the molecular classification of gliomas. Gene coexpression modules around epidermal growth factor receptor (EGFR) (EM, 29 genes) or platelet derived growth factor receptor A (PDGFRA) (PM, 40 genes) in gliomas were identified. Based on EM and PM expression signatures, nonnegative matrix factorization reproducibly clustered 1,369 adult diffuse gliomas WHO grades II-IV from four independent databases generated in three continents, into the subtypes (EM, PM and EMlowPMlow gliomas) in a morphology-independent manner. Besides their distinct patterns of genomic alterations, EM gliomas were associated with higher age at diagnosis, poorer prognosis, and stronger expression of neural stem cell and astrogenesis genes. Both PM and EMlowPMlow gliomas were associated with younger age at diagnosis and better prognosis. PM gliomas were enriched in the expression of oligodendrogenesis genes, whereas EMlowPMlow gliomas were enriched in the signatures of mature neurons and oligodendrocytes. The EM/PM-based molecular classification scheme is applicable to adult low-grade and high-grade diffuse gliomas, and outperforms existing classification schemes in assigning diffuse gliomas to subtypes with distinct transcriptomic and genomic profiles. The majority of the EM/PM classifiers, including regulators of glial fate decisions, have not been extensively studied in glioma biology. Subsets of these classifiers were coexpressed in mouse glial precursor cells, and frequently amplified or lost in an EM/PM glioma subtypespecific manner, resulting in somatic copy number alteration-dependent gene expression that contributes to EM/PM signatures in glioma samples. EM/PM-based molecular classification provides a molecular diagnostic framework to expedite the search for new glioma therapeutic targets.

Keywords

  • Cell and Molecular Biology

Other

Published
  • Rausing laboratory of Lund - Tumor section
  • ISSN: 0027-8424
Thoas Fioretos
E-mail: thoas.fioretos [at] med.lu.se

Principal investigator

Division of Clinical Genetics

+46 46 222 45 95

+46 70 334 33 67

BMC C13

66