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Expression of NUP98/TOP1, but not of TOP1/NUP98, in a treatment-related myelodysplastic syndrome with t(10;20;11)(q24;q11;p15).

Author:
  • Ioannis Panagopoulos
  • Thoas Fioretos
  • Margareth Isaksson
  • Gun Larsson
  • Rolf Billström
  • Felix Mitelman
  • Bertil Johansson
Publishing year: 2002
Language: English
Pages: 249-254
Publication/Series: Genes, Chromosomes and Cancer
Volume: 34
Issue: 2
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

The t(11;20)(p15;q11) is a rare but recurrent translocation that so far has been described in only four acute myeloid leukemias (AMLs), two treatment-related myelodysplastic syndromes (t-MDSs), and one case of polycythemia vera. Recently, the t(11;20) was shown to result in a fusion of the NUP98 and TOP1 genes, with expression of the NUP98/TOP1 chimera encoded by the der(11)t(11;20), but not of the reciprocal TOP1/NUP98 on the der(20)t(11;20). The genomic breakpoints were subsequently mapped to introns 13 and 7 of NUP98 and TOP1, respectively. We present here a t-MDS with a three-way variant translocation, t(10;20;11)(q24;q11;p15), that generates a der(11)t(11;20) but not a der(20)t(11;20), strongly suggesting that the der(11) harbors the critical genetic rearrangement. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed a NUP98/TOP1 fusion in which exon 13 of NUP98 was fused in-frame with exon 8 of TOP1. Extra long (XL) genomic PCR and subsequent sequence analyses showed that the breakpoint in NUP98 occurred at nucleotide (nt) 3461 of intron 13, close to a MER (medium reiteration frequency interspersed repetitive element) repeat, and that the breakpoint in TOP1 was at nt 1436 of intron 7, downstream of a MIR (mammalian-wide interspersed repeats) repetitive element. Genomic XL PCR did not amplify the reciprocal TOP1/NUP98, nor was this chimera expressed, as expected from the cytogenetic finding. The present results provide further support for the involvement of the NUP98/TOP1 transcript, but not of the reciprocal one, in the development of MDS/AML. Furthermore, the three cases genomically characterized to date have all been treatment-related and have all harbored breakpoints in intron 13 of NUP98 and intron 7 of TOP1, suggesting that these introns are susceptible to chemotherapy-induced breakage.

Keywords

  • Medical Genetics
  • Chromosomes
  • Human
  • Pair 20 : genetics
  • DNA Topoisomerases
  • Type I : biosynthesis
  • Type I : genetics
  • Female
  • Middle Age
  • Molecular Sequence Data
  • Myelodysplastic Syndromes : chemically induced
  • Myelodysplastic Syndromes : genetics
  • Nuclear Pore Complex Proteins : biosynthesis
  • Neoplasm Proteins : genetics
  • Nuclear Pore Complex Proteins : genetics
  • Support
  • Non-U.S. Gov't
  • Translocation (Genetics) : genetics
  • Pair 11 : genetics
  • Pair 10 : genetics
  • Human : genetics
  • Case Report
  • Base Sequence : genetics
  • Amino Acid Sequence : genetics

Other

Published
  • ISSN: 1045-2257
Thoas Fioretos
E-mail: thoas.fioretos [at] med.lu.se

Principal investigator

Division of Clinical Genetics

+46 46 222 45 95

+46 70 334 33 67

BMC C13

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