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Neuroblastoma Patient-Derived Orthotopic Xenografts Retain Metastatic Patterns and Geno- and Phenotypes of Patient Tumours.

Author:
  • Noémie Braekeveldt
  • Caroline Wigerup
  • David Gisselsson Nord
  • Sofie Mohlin
  • My Merselius
  • Siv Beckman
  • Tord Jonson
  • Anna Börjesson
  • Torbjörn Backman
  • Irene Tadeo
  • Ana P Berbegall
  • Ingrid Øra
  • Samuel Navarro
  • Rosa Noguera
  • Sven Påhlman
  • Daniel Bexell
Publishing year: 2015
Language: English
Pages: 252-261
Publication/Series: International Journal of Cancer
Volume: 136
Issue: 5
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

Neuroblastoma is a childhood tumour with heterogeneous characteristics and children with metastatic disease often have a poor outcome. Here we describe the establishment of neuroblastoma patient-derived xenografts (PDXs) by orthotopic implantation of viably cryopreserved or fresh tumour explants of patients with high risk neuroblastoma into immunodeficient mice. In vivo tumour growth was monitored by magnetic resonance imaging and fluorodeoxyglucose - positron emission tomography. Neuroblastoma PDXs retained the undifferentiated histology and proliferative capacity of their corresponding patient tumours. The PDXs expressed neuroblastoma markers NCAM, chromogranin A, synaptophysin and tyrosine hydroxylase. Whole genome genotyping array analyses demonstrated that PDXs retained patient-specific chromosomal aberrations such as MYCN amplification, deletion of 1p, and gain of chromosome 17q. Thus, neuroblastoma PDXs recapitulate the hallmarks of high-risk neuroblastoma in patients. PDX-derived cells were cultured in serum-free medium where they formed free-floating neurospheres, expressed neuroblastoma gene markers MYCN, CHGA, TH, SYP and NPY, and retained tumour-initiating and metastatic capacity in vivo. PDXs showed much higher degree of infiltrative growth and distant metastasis as compared to neuroblastoma SK-N-BE(2)c cell line-derived orthotopic tumours. Importantly, the PDXs presented with bone marrow involvement, a clinical feature of aggressive neuroblastoma. Thus, neuroblastoma PDXs serve as clinically relevant models for studying and targeting high-risk metastatic neuroblastoma. © 2014 Wiley Periodicals, Inc.

Keywords

  • Cancer and Oncology

Other

Published
  • CREATE Health
  • Molecular Pediatric Oncology
  • ISSN: 0020-7136
Sven Påhlman
E-mail: sven.pahlman [at] med.lu.se

Professor

Division of Translational Cancer Research

+46 46 222 64 21

MV406 312K1

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