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Scintigraphic method to quantify the passage from brain parenchyma to the deep cervical lymph nodes in rats

Author:
  • Håkan Widner
  • Bo-Anders Jönsson
  • Lars Hallstadius
  • Karin Wingårdh
  • Sven-Erik Strand
Publishing year: 1987
Language: English
Pages: 456-461
Publication/Series: European Journal of Nuclear Medicine and Molecular Imaging
Volume: 13
Issue: 9
Document type: Journal article
Publisher: Springer

Abstract english

In order to investigate the kinetics of the passage from the brain parenchyma to the lymphatic system in vivo a high resolution scintillation camera technique was developed. Albumin, albumin colloids and dextran labelled with 99mTc were injected into the right side of the thalamus of anesthetized rats. Continuous measurement of the activity distribution in head and neck were performed for 70 min. Rate constants were calculated by means of a computer program for compartment analysis. The animals were killed 10 h postinjection, to measure the activity in tissue samples. For some animals, images were registered up to 24 h after injection. This work presents an in vivo technique to clarify the particle flow paths in the brain and make quantification possible. The method is simple and does not require continuous blood or lymph sampling. Our work shows that there is a substantial flow of injected material from the brain via lamina cribriformis to the lymphatic system. This route is of great interest for the drainage of the interstitial fluid of the brain. We have also shown a difference in flow for dextran particles with different charge

Keywords

  • Radiology, Nuclear Medicine and Medical Imaging
  • Cervical lymph nodes
  • Lymph flow
  • Compartment analysis
  • Colloids
  • Dextran

Other

Published
  • ISSN: 1619-7070
Sven-Erik Strand
E-mail: sven-erik.strand [at] med.lu.se

Project manager

Systemic Radiation Therapy Group

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Professor emeritus

Medical Radiation Physics, Lund

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