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A nonsurgical technique for blood access in extracorporeal affinity adsorption of antibodies in rats.

Author:
  • Linda Mårtensson
  • Rune Nilsson
  • Hans Olov Sjögren
  • Sven-Erik Strand
  • Jan Tennvall
Publishing year: 2007
Language: English
Pages: 312-316
Publication/Series: Artificial Organs
Volume: 31
Issue: 4
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

Monoclonal antibodies for targeting cytotoxic conjugates to tumor cells are currently being evaluated together with extracorporeal affinity adsorption. The aim of the adsorption was to reduce undesired side effects in normal organs and to increase the tumor-to-normal tissue ratios. This technique is also applicable to several other therapeutic areas such as immune-mediated disorders, that is, autoimmunity, allergy, and transplantation rejection. We describe an improved technique for extracorporeal affinity adsorption of radiolabeled biotinylated antibodies in rats. Blood access is established through the tail artery and tail vein, without surgical insertion of permanent catheters. This technique is simple, does not require surgery, and causes only minimal stress to the animals. In addition, experiments can be carried out on several animals simultaneously. This new technique is of considerable benefit for studying extracorporeal affinity adsorption in rats, as experiments can be carried out with negligible anatomical and physiological interventions, compared to previously used techniques.

Keywords

  • Surgery
  • Radiology, Nuclear Medicine and Medical Imaging
  • Cancer and Oncology
  • Neurology
  • adsorption
  • radioimmunotherapy
  • affinity
  • blood access
  • extracorporeal
  • rat
  • avidin-biotin system
  • immunotargeting
  • immune-mediated disorders

Other

Published
  • ISSN: 0160-564X
Sven-Erik Strand
E-mail: sven-erik.strand [at] med.lu.se

Project manager

Systemic Radiation Therapy Group

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Professor emeritus

Medical Radiation Physics, Lund

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