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Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method

Author:
  • Michael Garkavij
  • Jan Tennvall
  • Sven-Erik Strand
  • Rune Nilsson
  • Lars Lindgren
  • Jangin Chen
  • Martin Isaksson
  • Håkan Eriksson
  • Hans-Olof Sjögren
Publishing year: 1996
Language: English
Pages: 309-312
Publication/Series: Acta Oncologica
Volume: 35
Issue: 3
Document type: Journal article
Publisher: Taylor & Francis

Abstract english

This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable.

Keywords

  • Cancer and Oncology

Other

Published
  • ISSN: 1651-226X
Sven-Erik Strand
E-mail: sven-erik.strand [at] med.lu.se

Project manager

Systemic Radiation Therapy Group

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Professor emeritus

Medical Radiation Physics, Lund

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