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Peter James

Peter James

Professor

Peter James

Glycolytic enzyme expression and pyruvate kinase activity in cultured fibroblasts from type 1 diabetic patients with and without nephropathy

Author

  • E Iori
  • R Millioni
  • L Puricelli
  • Giorgio Arrigoni
  • L Lenzini
  • R Trevisan
  • Peter James
  • GP Rossi
  • LA Pinna
  • P Tessari

Summary, in English

Since type 1 diabetes mellitus (T1DM) patients with nephropathy (DN+) are insulin-resistant, we aimed to identify (new) potential molecular sites involved in the alterations of glucose metabolism in these patients. We examined the expression of glycolytic enzymes in cultured fibroblasts from T1DM(DN+) patients as compared to those from T1DM patients without nephropathy (DN-) and from controls. Pyruvate kinase (PK) activity was also determined. Human skin fibroblasts were grown in normal glucose (6 mM), RNAs and proteins were analyzed, respectively, using cRNA microarray and two-dimensional electrophoresis followed by identification with mass spectrometry. PK activity was measured using a spectrophotometric assay. As compared to controls, increases in the gene expression of hexokinase, phosphoglucomutase, phosphofructokinase, aldolase and triosephosphate isomerase were found in T1DM(DN+) patients, but not in T1DM(DN-) patients. In T1DM(DN+) patients, the protein analysis showed an altered expression of three glycolytic enzymes: triosophosphate isomerase, enolase and PK. In addition, PK activity in fibroblasts from T1DM(DN+) patients was lower than that in T1DM(DN-) and in controls. In conclusion, this study reports novel alterations of enzymes involved in glucose metabolism that may be associated with the pathophysiology of insulin resistance and of renal damage in T1DM(DN+) patients.

Department/s

  • Department of Immunotechnology

Publishing year

2008

Language

English

Pages

627-633

Publication/Series

Biochimica et Biophysica Acta - Molecular Basis of Disease

Volume

1782

Issue

11

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 0925-4439