Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Proteomic analysis and discovery using affinity proteomics and mass spectrometry.

Author:
  • Niclas Olsson
  • Christer Wingren
  • Mikael Mattsson
  • Peter James
  • David O'Connell
  • Fredrik Nilsson
  • Dolores J Cahill
  • Carl Borrebaeck
Publishing year: 2011
Language: English
Publication/Series: Molecular & Cellular Proteomics
Volume: 10
Document type: Journal article
Publisher: American Society for Biochemistry and Molecular Biology

Abstract english

Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept.

Keywords

  • Immunology in the medical area

Other

Published
  • CREATE Health
  • ISSN: 1535-9484
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

MV406411E1

90