Peter James
Professor
Quantification of Membrane Proteins Using Nonspecific Protease Digestions
Author
Summary, in English
We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
Department/s
- Molecular Cell Biology
- Department of Immunotechnology
Publishing year
2009
Language
English
Pages
5666-5673
Publication/Series
Journal of Proteome Research
Volume
8
Issue
12
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Immunology in the medical area
Keywords
- membrane proteins
- N-terminal labeling
- quantification
- relative
- proteinase K
- Bacillus subtilis
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893