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Peter James

Peter James

Professor

Peter James

Quantification of Membrane Proteins Using Nonspecific Protease Digestions

Author

  • Maria Bendz
  • Mirja Carlsson Möller
  • Giorgio Arrigoni
  • Åsa Wåhlander
  • Roberto Stella
  • Salvatore Cappadona
  • Fredrik Levander
  • Lars Hederstedt
  • Peter James

Summary, in English

We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.

Department/s

  • Molecular Cell Biology
  • Department of Immunotechnology

Publishing year

2009

Language

English

Pages

5666-5673

Publication/Series

Journal of Proteome Research

Volume

8

Issue

12

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Immunology in the medical area

Keywords

  • membrane proteins
  • N-terminal labeling
  • quantification
  • relative
  • proteinase K
  • Bacillus subtilis

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3893