Peter James
Professor
Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B.
Author
Summary, in English
Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE hepatoma cells whereas S277 and S507 were phosphorylated in hepatoma cells only. Several of the sites were phosphorylated by insulin as well as cAMP-increasing hormones indicating integration of the two signalling pathways upstream of PDE3B, maybe at the level of protein kinase B.
Department/s
- Insulin Signal Transduction
- Department of Immunotechnology
Publishing year
2007
Language
English
Pages
584-592
Publication/Series
Biochimica et Biophysica Acta: Molecular Cell Research
Volume
1773
Issue
4
Full text
- Available as PDF - 892 kB
- Download statistics
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Endocrinology and Diabetes
Keywords
- Phosphorylation
- PDE3B
- Adipocyte
- Insulin
- Hepatocyte
- cAMP
Status
Published
Research group
- Insulin Signal Transduction
ISBN/ISSN/Other
- ISSN: 0167-4889