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Protein expression analysis: from "tip of the iceberg" to a global method.

Author:
  • Peter James
Publishing year: 2001
Language: English
Pages: 235-246
Publication/Series: Disease Markers
Volume: 17
Issue: 4
Document type: Journal article
Publisher: IOS Press

Abstract english

In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings.

Keywords

  • Medical Engineering
  • Gel
  • Two-Dimensional : trends
  • Genome
  • Proteins : metabolism
  • Electrophoresis

Other

Published
  • ISSN: 0278-0240
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

MV406411E1

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