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Biosynthesis of selenoproteins in cultured bovine mammary cells.

Author:
  • Katharina Bruzelius
  • S Purup
  • Peter James
  • Gunilla Önning
  • Björn Åkesson
Publishing year: 2008
Language: English
Pages: 224-233
Publication/Series: Journal of Trace Elements in Medicine and Biology
Volume: 22
Issue: 3
Document type: Journal article
Publisher: Elsevier

Abstract english

The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the system, the effect of retinoic acid (RA) on the formation of selenoproteins was investigated. The concentration of 75Se in total cell protein was reduced by about 35% in cells cultured with RA compared with control cells, while the opposite effect was observed in protein precipitated from cell culture medium, which contained 60% more 75Se in RA-treated samples than in controls. There were also indications that RA might affect different selenoproteins in different ways. The methods described provide a promising approach for further studies of the regulation of selenoprotein formation in the mammary gland.

Keywords

  • Immunology in the medical area
  • Biochemistry and Molecular Biology

Other

Published
  • Carl Borrebaeck-lup-obsolete
  • ISSN: 1878-3252
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

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+46 70 247 79 60

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