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Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis

Author:
  • Paolo Cifani
  • Maria Bendz
  • Kristofer Wårell
  • Karin M Hansson
  • Fredrik Levander
  • Marianne Sandin
  • Morten Krogh
  • Marie Ovenberger
  • Erik Fredlund
  • Marica Vaapil
  • Alexander Pietras
  • Sven Påhlman
  • Peter James
Publishing year: 2011
Language: English
Pages: 1645-1656
Publication/Series: Journal of Proteome Research
Volume: 10
Issue: 4
Document type: Journal article
Publisher: The American Chemical Society

Abstract english

Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.

Keywords

  • Biophysics
  • Immunology in the medical area
  • isotopic labeling
  • HPLC-MS
  • 2D-PAGE
  • plasma membrane
  • protein
  • expression
  • mRNA expression
  • correlation
  • hypoxia
  • normoxia
  • neuroblastoma

Other

Published
  • ISSN: 1535-3893
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

MV406411E1

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