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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Author:
  • Åsa Wåhlander
  • Giorgio Arrigoni
  • Kristofer Wårell
  • Fredrik Levander
  • Ronnie Palmgren
  • Jean-Luc Maloisel
  • Philippe Busson
  • Peter James
Publishing year: 2007
Language: English
Pages: 1101-1113
Publication/Series: Journal of Proteome Research
Volume: 6
Issue: 3
Document type: Journal article
Publisher: The American Chemical Society

Abstract english

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

Keywords

  • Immunology in the medical area
  • precursor-ion
  • reagents
  • mass spectrometry
  • isotope labeling
  • HPLC
  • parent-ion

Other

Published
  • ISSN: 1535-3893
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

MV406411E1

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