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Peter James

Peter James

Professor

Peter James

Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.

Author

  • Barbara Valastro
  • Andrzej Dekundy
  • Morten Krogh
  • Martin Lundblad
  • Peter James
  • Wojciech Danysz
  • Guenter Quack
  • Angela Cenci Nilsson

Summary, in English

L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID.

Department/s

  • Basal Ganglia Pathophysiology
  • Computational Biology and Biological Physics - Undergoing reorganization
  • Departments at LTH

Publishing year

2007

Language

English

Pages

1395-1409

Publication/Series

Journal of Neurochemistry

Volume

102

Issue

4

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Neurosciences

Keywords

  • spectrometry
  • two-dimensional difference gel electrophoresis
  • Parkinson's disease
  • mass
  • levodopa
  • dyskinesia
  • bromocriptine

Status

Published

Research group

  • Basal Ganglia Pathophysiology

ISBN/ISSN/Other

  • ISSN: 1471-4159