Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.

Author:
  • Barbara Valastro
  • Andrzej Dekundy
  • Morten Krogh
  • Martin Lundblad
  • Peter James
  • Wojciech Danysz
  • Guenter Quack
  • Angela Cenci Nilsson
Publishing year: 2007
Language: English
Pages: 1395-1409
Publication/Series: Journal of Neurochemistry
Volume: 102
Issue: 4
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID.

Keywords

  • Neurosciences
  • spectrometry
  • two-dimensional difference gel electrophoresis
  • Parkinson's disease
  • mass
  • levodopa
  • dyskinesia
  • bromocriptine

Other

Published
  • Basal Ganglia Pathophysiology
  • ISSN: 1471-4159
Peter James
E-mail: peter.james [at] immun.lth.se

Professor

Department of Immunotechnology

+46 46 222 14 96

+46 70 247 79 60

MV406411E1

90